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Three plasmids (pGL1124, pGL1224 and pGL1217) ended up made to enable the substitute of one allele of CYC9Lister 427 cells. Trypanosomes were being transfected with Just about every plasmid separately, after which you can subjected to a next transfection with one of the other plasmids, with a different resistance gene, with all pairwise combos exec